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Item 1 of 5
Item 1 of 5
GPNMB Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1173109
| Catalog Number | orb1173109 |
|---|---|
| Category | Antibodies |
| Description | GPNMB Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | F8D5 |
| Tested applications | FC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Human, Mouse |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-1:1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-1:100 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| Target | GPNMB |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: A375, 2: B16-F0, 3: EL4.IL-2, 4: HeLa, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 64 kDa, Observed MW: 120, 95, 65 kDa.
Cell line: A375, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1173109.
Cell line: EL4, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1173109.
Tissue: Human tonsil, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173109.
Tissue: Mouse small intestine, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173109.
