GPI / Neuroleukin Antibody

• Catalog Number: orb1248598
• Category: Antibodies
• Description: GPI / Neuroleukin Antibody
• Target: GPI / Neuroleukin
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: The immunogen for this antibody is: C-RERMFNGEKINYT
• UniProt ID: P06744
• MW: Approx 60kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Heart lysates and approx 55kDa observed in lysates of cell lines HeLa, A431, A549, MCF7, Jurkat and K562 (calculated MW of 63.1kDa according to NP_000166.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis, Thyroid and Kidney. Recommended concentration: 3.75ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GPI, glucose phosphate isomerase, AMF, GNPI, NLK,
• Note: For research use only
• NCBI: NP_000166.2
• Expiration Date: 12 months from date of receipt.

orb1248598 (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248598 (0.05 ug/ml) staining of HeLa (A), A431 (B), A549 (C), MCF7 (D), Jurkat (E) and K562 (F) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248598 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248598 (3.75 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

orb1248598 (3.75 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.