GPI/Neuroleukin Antibody

SKU: orb1248598

Description

GPI/Neuroleukin Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Heart lysates and approx 55kDa observed in lysates of cell lines HeLa, A431, A549, MCF7, Jurkat and K562 (calculated MW of 63.1kDa according to NP_000166.2). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis, Thyroid and Kidney. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RERMFNGEKINYT
• Target: GPI / Neuroleukin
• Molecular Weight: Approx 60kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GPI, glucose phosphate isomerase, AMF, GNPI, NLK, PGI, PHI, SA-36, autocrine motility factor, glucose-6-phosphate isomerase, hexose monophosphate isomerase, hexosephosphate isomerase, neuroleukin, oxoisomerase, phosphoglucose isomerase, phosphohexomutase, phosphohexose isomerase, phosphosaccharomutase, sperm antigen-36

orb1248598 (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248598 (0.05 ug/ml) staining of HeLa (A), A431 (B), A549 (C), MCF7 (D), Jurkat (E) and K562 (F) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248598 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248598 (3.75 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

orb1248598 (3.75 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

GPI / Neuroleukin

UniProt

P06744

RefSeq (Protein):NP_000166.2