GPI Antibody / Glucose-6-phosphate isomerase

• Catalog Number: orb639688
• Category: Antibodies
• Description: In addition to it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-GPI-1
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A recombinant human full-length protein was used as the immunogen for the Glucose 6-Phosphate Isomerase antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Glucose 6-Phosphate Isomerase antibody is available for research use only.
• UniProt ID: P06744
• Storage: Store the Glucose 6-Phosphate Isomerase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast carcinoma with Glucose 6-Phosphate Isomerase antibody (clone CPTC-GPI-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Immunofluorescence staining of fixed human MCF-7 cells with Glucose 6-Phosphate Isomerase antibody (green, clone CPTC-GPI-1) and Reddot nuclear stain (red).

Western blot testing of human T-47D, PC3 and HePG2 cell lysate with Glucose 6-Phosphate Isomerase antibody. Predicted molecular weight: ~63 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Glucose 6-Phosphate Isomerase antibody (clone CPTC-GPI-1). These results demonstrate the foremost specificity of the CPTC-GPI-1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Glucose 6-Phosphate Isomerase antibody (clone CPTC-GPI-1) as confirmation of integrity and purity.