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Item 1 of 4
Item 1 of 4
GPD1L Antibody (N-term)
Catalog Number: orb1938757
| Catalog Number | orb1938757 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB28552 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50, WB: 1:2000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 38419 Da |
| Target | This GPD1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-73 amino acids from the N-terminal region of human GPD1L. |
| UniProt ID | Q8N335 |
| NCBI | NP_055956.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Glycerol-3-phosphate dehydrogenase 1-like protein, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-GPD1L Antibody (N-term) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-GPD1L Antibody (N-term) at 1:1000 dilution. Lane 1: Rat brain tissue lysate. Lane 2: Mouse thymus tissue lysate. Lane 3: Mouse heart tissue lysate. Lane 4: 293T cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size: 100kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
GPD1L Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GPD1L antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPD1L antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
