GPD1L Antibody (N-term)

• Catalog Number: orb1938757
• Category: Antibodies
• Description: GPD1L Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q8N335
• MW: 38419 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB - 1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: KIAA0089
• Research Area: Cell Biology, Metabolism Research, Signal Transduc
• Note: For research use only

All lanes: Anti-GPD1L Antibody (N-term) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GPD1L Antibody (N-term) at 1:1000 dilution. Lane 1: Rat brain tissue lysate. Lane 2: Mouse thymus tissue lysate. Lane 3: Mouse heart tissue lysate. Lane 4: 293T cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.Observed band size: 100kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

GPD1L Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

GPD1L antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPD1L antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.