GOT1 Antibody (N-term)

SKU: orb1931316

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Equine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD42941
• Immunogen: This GOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human GOT1. Antigen Region: 5-33 aa.
• Target: GOT1 (HGNC:4432)
• Molecular Weight: 46248 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Aspartate aminotransferase, cytoplasmic, cAspAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, cCAT, Glutamate oxaloacetate transaminase 1, Transaminase A, GOT1

Western blot analysis of GOT1 Antibody (N-term) in mouse brain tissue lysates (35 ug/lane). GOT1 (arrow) was detected using the purified Pab.

Western blot analysis of GOT1 (arrow) using rabbit polyclonal GOT1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GOT1 gene (Lane 2).

Anti-GOT1 Antibody (N-term) at 1:1000 dilution + human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

GOT1 (HGNC:4432)

UniProt

P17174

RefSeq (Protein):NP_002070.1