Gossypol (acetic acid)

SKU: orb1305100

Description

Gossypol acetic acid (AT101) is a cottonseed-derived polyphenol that functions as a pan-Bcl-2 inhibitor by binding to Bcl-2, Bcl-xL, and Mcl-1, while sparing the BIR3 domain and BID. It is widely used in cancer research, particularly in studies of apoptosis, with applications in both in vitro and in vivo experimental models.

Research Area

Cell Biology

Key Properties

• CAS Number: 12542-36-8
• MW: 578.61
• Purity: 98.09%
• Formula: C₃₂H₃₄O₁₀
• SMILES: CC(O)=O.CC(C)c1c(O)c(O)c(C=O)c2c(O)c(c(C)cc12)-c1c(C)cc2c(C(C)C)c(O)c(O)c(C=O)c2c1O
• Target: Bcl-2 Family
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:2 mg/mL (3.46 mM);DMSO:100 mg/mL (172.83 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (6.91 mM)

Bioactivity

• Target IC50:
  BCL-XL:0.5-0.6 μM (Ki)|Bcl-2:0.2-0.3 mM (Ki)
• In Vivo:
  Gossypol acetate is able to inhibit tumor growth in Wus1-bearing mice, but the survival of mice is not prolonged, and tumor grows rapidly after short inhibition. Gossypol has now been found to have inhibitory effects on proliferation or to induce apoptosis in ovarian cancer, endometrial cancer, adrenal cortical tumor, thyroid cancer, lung cancer, colon carcinoma, leukemia, pancreatic cancer, melanoma and lymphoma. In addition, gossypol can increase the sensitivity of drug-resistant tumor cells to chemotherapy and radiotherapy. Some Clinicalal trials showed gossypol is well-tolerated, and partial responses are observed in some patients.
• In Vitro:
  Gossypol, a known antispermatogenic agent from the cotton plant genus Gossypium, was found to inhibit yellow perch sperm motility in vitro and lactate dehydrogenase activity in spermatozoa when used in a dose-dependent manner. Gossypol has been approved to have antiproliferative and apoptosis-inducing effects on some kinds of cancer cell lines in vitro.
• Cell Research:
  MM(Multiple myeloma) cells are plated in 24-cell culture clusters at a density of 1x105 viable cells/l per well. Triplicate wells are treated with 1, 5, 10, 25 and 50 µmol/l gossypol acetate, and the negative control group is supplemented with 0.1% DMSO. Then, cell numbers at different treatment time points (0, 24, 48 and 72 h) are determined by using a hemocytometer and the trypan blue dye-exclusion method. The trypan blue dye-exclusion method is used to evaluate the cell viability. The cells are examined in a counting chamber under a light microscope. Only viable cells are recorded. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pogosin, inhibit, Gossypol, Gossypol (acetic acid), Gossypol acetic acid, Gossypol-acetic acid, Inhibitor, acetic acid, (±)-Gossypol-acetic acid, Bcl-2, Bcl-2 Family, BL-193 (acetic acid), AT101, AT-101, AT 101

Compound Identifiers

PubChem CID

227456