Golgi Marker Antibody [GLG1/970]

• Catalog Number: orb1252545
• Category: Antibodies
• Description: Golgi Marker Antibody [GLG1/970]
• Target: GLG1
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody.
• UniProt ID: Q92896
• Tested applications: FC, ICC, IF, IHC-P, WB
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 ug/ml for 30 minWestern blot: 0.5-1.0 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Golgi Marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: GLG1/970
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Golgi apparatus protein 1, CFR-1, Cysteine-rich fi
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human pancreas stained with Golgi Marker antibody (GLG1/970).