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Goat IL-6 (aa138-148) Antibody
Catalog Number: orb2899326
| Catalog Number | orb2899326 |
|---|---|
| Category | Antibodies |
| Description | Goat IL-6 (aa138-148) Antibody |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | Peptide with sequence C-EQARAVQMSTK, from the internal region of the protein sequence according to NP_000591.1; NP_001358025.1; NP_001305024.1. |
| Tested applications | ELISA, IF |
| Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 5µg/ml. Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml. |
| Storage | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | B cell stimulatory factor-2, B-cell differentiatio Read more... |
| Note | For research use only |
| NCBI | NP_001358025.1, NP_000591.1, NP_001305024.1 |
Immunofluorescence analysis of paraformaldehyde fixed U2OS. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic, nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
