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Catalog Number | orb346600 |
---|---|
Category | Antibodies |
Description | Goat IgG F(c) antibody |
Species/Host | Donkey |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Goat |
Isotype | IgG |
Immunogen | Goat IgG F(c) fragment |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Alternative names | donkey anti-Goat IgG F(c) fragment Antibody, donke Read more... |
Note | For research use only |
Application notes | Anti-Goat IgG F(c) antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
DOT, SDS-PAGE | |
TRITC | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat IgG, anti-Goat IgG F(c) and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(ab’)2 or anti-Papain. | |
Goat |
SDS-PAGE | |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Goat IgG and anti-Goat IgG F(c). No reaction was observed against anti-Goat IgG F(ab’)2 or anti-Papain. | |
Goat |
SDS-PAGE | |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat IgG, anti-Goat IgG F(c) and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(ab’)2 or anti- Papain. | |
Goat |
SDS-PAGE | |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat IgG, anti-Goat IgG F(c) and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(ab’)2 or anti-Papain. | |
Goat |
SDS-PAGE | |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat IgG, anti-Goat IgG F(c) and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(ab’)2 or anti-Papain. | |
Goat |
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