Goat Ig anti Human IgA (Fc specific) immunofixation

• Catalog Number: orb21782
• Category: Antibodies
• Description: Goat polyclonal antibody to Immunofixation.
• Species/Host: Goat
• Clonality: Polyclonal
• Antibody Type: Secondary Antibody
• Form/Appearance: Goat Ig lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information.
• Source: Highly purified polyclonal IgA and monoclonal IgA2 isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• RRID: AB_10752872
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Goat Ig lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
• Background: The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. This antiserum is intended to detect and identify IgA in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
• Note: For research use only
• Application notes: Immunoprecipitation.
• Expiration Date: 12 months from date of receipt.