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Goat CD163 Antibody
SKU: orb2899324
Description
Research Area
Immunology & Inflammation; Cell Biology
Images & Validation
−| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 150kDa band observed in lysates of cell line Caco-2 (calculated MW of 125kDa according to NP_004235.4). Molecular weights of 150-200kDa have been observed by other commercial sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. Flow Cytometry: Flow cytometric analysis of Caco-2 cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human |
Key Properties
−| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
|---|---|
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD163, CD163 molecule, M130, MM130, hemoglobin scavenger receptor, macrophage-associated antigen, scavenger receptor cysteine-rich type 1 protein M130

Quality Guarantee
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Quick Database Links
RefSeq (Protein):NP_981961.2, NP_004235.4
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_981961.2, NP_004235.4 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)


