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Catalog Number | orb19127 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ZNF703 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Canine, Human, Porcine |
Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 58.2 |
Target | ZNF703 |
Entrez | 80139 |
Protein Sequence | ELDKKDQEPKPSPE |
RRID | AB_10753122 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ZNF703 antibody, anti zinc finger protein 703 Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 28-30kDa band in human breast lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.2kDa according to NP_079345.1. The 28-30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported An additional band of approx 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Human Breast cancer lysate using ZNF703 antibody