ZNF703 Antibody

SKU: orb19127

Description

Goat polyclonal antibody to ZNF703

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 28-30kDa band in human breast lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.2kDa according to NP_079345.1. The 28-30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported An additional band of approx 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ZNF703
• Protein Sequence: ELDKKDQEPKPSPE
• Molecular Weight: 58.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ZNF703 antibody, anti zinc finger protein 703 antibody, anti FLJ14299 antibody, anti ZNF503L antibody, anti hypothetical protein LOC80139 antibody

Western blot analysis of Human Breast cancer lysate using ZNF703 antibody

Quick Database Links

Gene Symbol

ZNF703

NCBI Gene ID

80139