Goat anti-Ymer / CCDC50 Antibody

• Catalog Number: orb19009
• Category: Antibodies
• Description: Goat polyclonal to Ymer
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Bovine, Canine, Human, Rat
• Dilution range: ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 5-10µg/ml
• Conjugation: Unconjugated
• MW: 56.3; 35.8
• Target: Ymer / CCDC50
• Entrez: 152137
• Protein Sequence: CFSKSESSHKGFHYK
• RRID: AB_10751261
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CCDC50 antibody, anti Ymer protein antibody,
• Note: For research use only
• Application notes: This antibody is expected to recognise both reported isoforms of human Ymer protein.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows strong staining in cytoplasm in select acinar cells. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave an approx 18kDa band in Human Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_848018.1 and 35.8kDa according to NP_777568.1. The 18kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Pancreas using Ymer antibody