Ymer/CCDC50 Antibody

SKU: orb19009

Description

Goat polyclonal to Ymer

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 5-10µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  This antibody is expected to recognise both reported isoforms of human Ymer protein.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows strong staining in cytoplasm in select acinar cells. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave an approx 18kDa band in Human Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_848018.1 and 35.8kDa according to NP_777568.1. The 18kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Ymer / CCDC50
• Protein Sequence: CFSKSESSHKGFHYK
• Molecular Weight: 56.3; 35.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CCDC50 antibody, anti Ymer protein antibody, anti coiled-coil domain containing 50 antibody, anti C3orf6, YMER antibody, anti C3orf6 antibody, anti chromosome 3 open reading frame 6 antibody, anti DFNA44 antibody

Immunohistochemical staining of Human Pancreas using Ymer antibody

Quick Database Links

Gene Symbol

Ymer / CCDC50

NCBI Gene ID

152137