Goat anti-XBP1 / TREB5 Antibody

• Catalog Number: orb19868
• Category: Antibodies
• Description: Goat polyclonal antibody to XBP1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:4000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 40.1; 28.7
• Target: XBP1 / TREB5
• Entrez: 7494
• Protein Sequence: NHSWEDTFANELFPQ
• RRID: AB_10750430
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti XBP1 antibody, anti TREB5 antibody, anti X-bo
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 38kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 40.1kDa according to NP_001073007.1). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19868 (1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19868 (5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.