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WNT15/WNT9B Antibody

SKU: orb20309

Description

Goat polyclonal antibody to WNT9B

Research Area

Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:8000, WB: 0.3-1 μg/ml
ReactivityHuman, Mouse
Predicted ReactivityRat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 38kDa band observed in Human Brain Cerebellum lysates (calculated MW of 39kDa according to NP_003387.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetWNT15 / WNT9B
Protein SequenceKRGNKDLRARADA
Molecular Weight39
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti WNT9B antibody, anti wingless-type MMTV integration site family, member 9B antibody, anti WNT14B antibody, anti WNT15 antibody, anti wingless-type MMTV integration site family, member 15 antibody

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WNT15/WNT9B Antibody

orb20309 (0.3ug/ml) staining of Human Brain Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WNT15/WNT9B Antibody

Western blot analysis of staining of Mouse Kidney (A) and Brain (B) lysate using WNT9B antibody.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

WNT15/WNT9B Antibody (orb20309)

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Available Sizes

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100 μg
$ 530.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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