VPS25 Antibody

SKU: orb19138

Description

Goat polyclonal antibody to VPS25

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  WB: Approx. 22kDa band observed in Rat Kidney lysates (calculated MW of 20.7kDa according to human NP_115729.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Kidney. Recommended concentration: 6-8µg/ml.

Key Properties

• Host: Goat
• Target: VPS25
• Protein Sequence: QPNVDTRQKQ
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VPS25; vacuolar protein sorting 25 (yeast); HGNC:28122; DERP9; EAP20; MGC10540; vacuolar protein sorting 25; vacuolar protein sorting 25 homolog (S. cerevisiae); FAP20

orb19138 (0.5ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19138 (10ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Quick Database Links

Gene Symbol

VPS25