Goat anti-Vitamin D-binding protein / GC Antibody

• Catalog Number: orb19850
• Category: Antibodies
• Description: Goat polyclonal antibody to GC
• Target: Vitamin D-binding protein / GC
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CDNLSTKNSKFED
• RRID: AB_10752185
• MW: 52.9
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 56kDa band observed in Human Lung lysates (calculated MW of 52.9kDa according to NP_000574.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (DiMartino and Kew, J Immunol. 1999 Aug 15;163(4):2135-42; PMID: 10438954). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti GC antibody, anti vitamin D-binding protein a
• Note: For research use only
• Entrez: 2638
• Expiration Date: 12 months from date of receipt.

orb19850 (0.03ug/ml) staining of Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.