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VHL Antibody

SKU: orb19313

Description

Goat polyclonal antibody to VHL

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:128000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman, Mouse, Rat
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 17kDa band observed in Human Ovary and Testis lysates and in lysates of all cell lines tested (calculated MW of 19.7kDa according to NP_937799.1). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

ClonalityPolyclonal
TargetVHL
Protein SequenceRSLVKPENYRRLD
Molecular Weight24.2; 19.7
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti VHL antibody, anti von Hippel-Lindau tumor suppressor antibody, anti HRCA1 antibody, anti RCA1 antibody, anti VHL1 antibody, anti elongin binding protein antibody, anti von Hippel-Lindau syndrome antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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