VEGFA (aa288-299) Antibody

SKU: orb12277

Description

Goat polyclonal antibody to VEGF

Research Area

Cancer

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  WB: Approx 45kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5kDa according to NP_001020537.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat (Liver, Kidney. IHC: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: VEGFA (aa288-299)
• Protein Sequence: RIKPHQGQHIGE
• Molecular Weight: 45.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Vascular endothelial growth factor A antibody, anti Vascular Endothelial Growth Factor antibody, anti Vascular Permeability Factor antibody, anti VEGF-A antibody, anti VEGFA antibody, anti VPF antibody

Western blot analysis of HEK293 lysate using VEGF antibody

Immunohistochemical staining of Human Liver using VEGF antibody

Immunohistochemical staining of Human Small Intestine using VEGF antibody

Quick Database Links

Gene Symbol

VEGFA (aa288-299)

NCBI Gene ID

7422