USP28 Antibody

• Catalog Number: orb19956
• Category: Antibodies
• Description: Goat polyclonal antibody to USP28
• Target: USP28
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SVELKHYIQEDN
• RRID: AB_10926103
• MW: 122
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:64000, WB: 0.2 μg/ml, IHC-P: 2.5-3.8µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 150kDa and 50kDa in Human Bone Marrow lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 122kDa according to NP_065937.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti USP28 antibody, anti ubiquitin specific pepti
• Research Area: Cancer, DNA Damage, Epigenetics
• Note: For research use only
• Entrez: 57646

Immunohistochemical staining of Human Testis using USP28 antibody