UPF3A/RENT3A Antibody

• Catalog Number: orb20368
• Category: Antibodies
• Description: Goat polyclonal antibody to UPF3A
• Target: UPF3A / RENT3A
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Predicted Reactivity: Bovine, Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KETDKQKKIAEKE
• RRID: AB_10750228
• MW: 54.7; 51.0
• Tested applications: ELISA
• Dilution range: ELISA: 1:8000, WB: 0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 48kDa band in lysates of cell lines HeLa and Kelly after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51kDa according to NP_542418.1 and the reported sizes of 65 to 70kDa from different sources. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti UPF3A antibody, anti UPF3 regulator of nonsen
• Note: For research use only
• Entrez: 65110