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Item 1 of 4
Goat anti-TRPM7 / LTRPC7 Antibody
Catalog Number: orb18297
| Catalog Number | orb18297 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to TRPM7 |
| Target | TRPM7 / LTRPC7 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Canine, Human, Mouse, Porcine, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | TKESESTNSVRLML |
| RRID | AB_10750064 |
| MW | 213; 213 |
| Tested applications | ELISA, IF, IHC |
| Dilution range | ELISA: 1:8000, IHC-P: 4-6 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 150kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 213kDa according to NP_060142.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Clark et al PLoS ONE. 2008 Mar 26;3(3):e1876.; PMID: 18365021). Recommended concentration: 0.3- 1 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti TRPM7 antibody, anti transient receptor poten Read more... |
| Note | For research use only |
| Entrez | 54822 |
Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
6 µg/ml staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
