Goat anti-TRIM4 Antibody

• Catalog Number: orb18515
• Category: Antibodies
• Description: Goat polyclonal to TRIM4
• Target: TRIM4
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ASLVIPPVTDRK
• RRID: AB_10755403
• MW: 57.5, 54.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 1 μg/ml
• Application notes: This antibody is expected to recognise both reported human isoforms (as represented by NP_148977 and NP_149082).ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55-60kDa band observed in HepG2 whole cell lysate (predicted MW of 58kDa and 55kDa according to NP_148977 and NP_149082). Recommended for use at 1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TRIM4 antibody, anti RNF87 antibody, anti tri
• Note: For research use only
• Entrez: 89122

orb18515 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.