TRIM2 Antibody

SKU: orb18436

Description

Goat polyclonal antibody to TRIM2

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.5-1 μg/ml, IHC-P: 6-12 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 80+85kDa bands observed in Rat Brain lysates (calculated MW of 81.5kDa according to Human NP_001123539.1, 84.4 accorindg to Human NP_056086.2 and 83.3 according to Rat NP_001102022.1). Recommended concentration: 0.5-2 μg/ml.. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TRIM2
• Protein Sequence: DSGNHCFKVYRYLQ
• Molecular Weight: 84.5; 81.5; 83.9; 83.6; 81.8; 67.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TRIM2 antibody, anti tripartite motif-containing 2 antibody, anti KIAA0517 antibody, anti tripartite motif protein 2 antibody, anti tripartite motif protein TRIM2 antibody, anti RNF86 antibody, anti tripartite motif protein TRIM2 antibody

Western blot analysis of Rat Brain lysate using TRIM2 antibody.

Immunohistochemical staining of Human Cortex using TRIM2 antibody

Quick Database Links

Gene Symbol

TRIM2

NCBI Gene ID

23321