TRAC-1/RNF125 Antibody

SKU: orb18811

Description

Goat polyclonal antibody to RNF125

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: ELISA: 1:8000, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave no signal but low background in Human Brain and Ovary lysates at up to 1 μg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TRAC-1 / RNF125
• Protein Sequence: RSLLEYVNHSNTT
• Molecular Weight: 26.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RNF125 antibody, anti FLJ20456 antibody, anti MGC21737 antibody, anti ring finger protein 125 antibody, anti TRAC1 antibody, anti T-cell ring protein identified in activation screen antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using RNF125 antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using RNF125 antibody.

Quick Database Links

Gene Symbol

TRAC-1 / RNF125

NCBI Gene ID

54941