TPPP/p25 Antibody

SKU: orb20600

Description

Goat polyclonal antibody to TPPP

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.001-0.01 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01μg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2µg/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: TPPP/p25
• Protein Sequence: DPSKDRAAKRLSLES
• Molecular Weight: 23.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti brain specific protein p25 alpha antibody, anti glycogen synthase kinase 3 (GSK3) inhibitor p24 antibody, anti p24 antibody, anti p25 antibody, anti p25alpha antibody, anti TPPP/p25 antibody, anti TPPP1 antibody, anti tubulin polymerization promoting protein antibody, anti tubulin polymerization-promoting protein antibody, anti TPPP antibody

Quick Database Links

Gene Symbol

TPPP/p25

NCBI Gene ID

11076