TPPP/p25 Antibody

SKU: orb20600

Description

Goat polyclonal antibody to TPPP

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.001-0.01 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01μg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2µg/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: TPPP/p25
• Protein Sequence: DPSKDRAAKRLSLES
• Molecular Weight: 23.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti brain specific protein p25 alpha antibody, anti glycogen synthase kinase 3 (GSK3) inhibitor p24 antibody, anti p24 antibody, anti p25 antibody, anti p25alpha antibody, anti TPPP/p25 antibody, anti TPPP1 antibody, anti tubulin polymerization promoting protein antibody, anti tubulin polymerization-promoting protein antibody, anti TPPP antibody

Immunohistochemical staining of Human Cerebellum using TPPP antibody

Immunohistochemical staining of Human Cerebellum using TPPP antibody

Immunohistochemical staining of Human Cortex using TPPP antibody

Western blot analysis of Mouse (Lane1) and Rat (Lane2) Brain lysate using TPPP antibody

Western blot analysis of Human Cerebellum lysate using TPPP antibody

Quick Database Links

Gene Symbol

TPPP/p25

NCBI Gene ID

11076