TPPP/p25 Antibody

• Catalog Number: orb20600
• Category: Antibodies
• Description: Goat polyclonal antibody to TPPP
• Target: TPPP/p25
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DPSKDRAAKRLSLES
• RRID: AB_10753900
• MW: 23.7
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.001-0.01 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01μg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2µg/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti brain specific protein p25 alpha antibody, an
• Note: For research use only
• Entrez: 11076

Immunohistochemical staining of Human Cerebellum using TPPP antibody

Immunohistochemical staining of Human Cerebellum using TPPP antibody

Immunohistochemical staining of Human Cortex using TPPP antibody

Western blot analysis of Mouse (Lane1) and Rat (Lane2) Brain lysate using TPPP antibody

Western blot analysis of Human Cerebellum lysate using TPPP antibody