TMP21/TMED10 Antibody

• Catalog Number: orb19451
• Category: Antibodies
• Description: Goat polyclonal antibody to TMED10
• Target: TMP21 / TMED10
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: YLRRFFKAKKLIE
• RRID: AB_10749367
• MW: 25
• Tested applications: ELISA, FC, IF
• Dilution range: ELISA: 1:1000, WB: 1 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments gave an approx 75kDa band in Human and Rat Pancreas lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to NP_006818.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TMED10 antibody, anti transmembrane emp24-lik
• Research Area: Cancer, Neuroscience
• Note: For research use only
• Entrez: 10972

Immunofluorescence analysis of U251 cells using TMED10 antibody

Flow cytometric analysis of HeLa cells using TMED10 antibody