Goat Anti-TLR6 Antibody

SKU: orb2657252

Description

Goat Anti-TLR6 Antibody

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:1000. Immunofluorescence: Strong expression of the protein seen in THP-1 and A549 cells. Recommended concentration: 10µg/ml.
• Reactivity: Human
• Predicted Reactivity: Bovine, Porcine, Sheep

Key Properties

• Host: Goat
• Immunogen: Peptide with sequence C-RGSTLLNFTLNH, from the internal region of the protein sequence according to NP_006059.2.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD286, OTTHUMP00000158730, toll-like receptor 6, TLR6

Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic (endoplasmic reticulum) staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

RefSeq (Protein):NP_006059.2