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Goat Anti-TLR6 Antibody
Catalog Number: orb2657252
| Catalog Number | orb2657252 |
|---|---|
| Category | Antibodies |
| Description | Goat Anti-TLR6 Antibody |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Porcine, Sheep |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | Peptide with sequence C-RGSTLLNFTLNH, from the internal region of the protein sequence according to NP_006059.2. |
| Tested applications | ELISA, IF |
| Dilution range | Peptide ELISA: antibody detection limit dilution 1:1000. Immunofluorescence: Strong expression of the protein seen in THP-1 and A549 cells. Recommended concentration: 10µg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CD286, OTTHUMP00000158730, toll-like receptor 6, T Read more... |
| Note | For research use only |
| NCBI | NP_006059.2 |
Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic (endoplasmic reticulum) staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
