TIM50 Antibody

SKU: orb18894

Description

Goat polyclonal antibody to TIMM50

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.5-1.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Experiments gave bands at approx 40kDa in Human Heart lysate after 0.5 μg/ml antibody staining. This band corresponds to earlier findings in literature with different antibodies (PMID: 15044455). This protein has a calculated MW of 50.5kDa according to NP_001001563. Recommended concentration: 0.5-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TIM50
• Protein Sequence: CDVRTVLEHYALEDD
• Molecular Weight: 50.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TIMM50 antibody, anti TIM50L antibody, anti translocase of inner mitochondrial membrane 50 homolog (yeast) antibody, anti Tim50-like protein antibody, anti homolog of yeast Tim50 antibody, anti translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody, anti MGC102733 antibody, anti TIM50 antibody

orb18894 staining (0.5ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

TIM50

NCBI Gene ID

92609