Thrombospondin Antibody

• Catalog Number: orb167695
• Category: Antibodies
• Description: Goat polyclonal antibody to THBS1.
• Target: Thrombospondin
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NRIPESGGDNSVFD
• MW: 129
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 150kDa band observed in Mouse Kidney lysates (calculated MW of 129kDa according to NP_035710.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti THBS1 antibody, anti thrombospondin 1 antibod
• Note: For research use only
• Entrez: 7057
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 lysate (10ug protein in RIPA buffer) using THBS1 antibody

Immunohistochemical staining of Human Spleen using THBS1 antibody

Immunohistochemical staining of Human Placenta using THBS1 antibody

Western blot analysis of Mouse Kidney lysate using THBS1 antibody

Immunohistochemical staining of Human Spleen using THBS1 antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using THBS1 antibody

Immunohistochemical staining of Human Spleen using THBS1 antibody

Flow cytometric analysis of paraformaldehyde fixed A431 cells using THBS1 antibody

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells using THBS1 antibody