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THNSL2 Antibody

SKU: orb79347

Description

Goat polyclonal antibody to THNSL2

Images & Validation

Tested ApplicationsELISA, IHC
Dilution rangeELISA: 1:128000, WB: 1 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine
Application Notes
WB: Preliminary experiments gave an approx 35kDa band in Human Colorectal Cancer lysates after 1μg/ml antibody staining.Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.0kDa according to NP_001231605.1 and 54.1kDa according to NP_060741.3.The 35kDa band was successfully blocked by incubation with the immunizing peptide.We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates.Have any further splice variants/modified forms been reported.Experiment Notes: This antibody is expected to recognize isoform 1 (NP_060741.3) and isoform 2 (NP_001231605.1) only.

Key Properties

HostGoat
ClonalityPolyclonal
TargetTHNSL2
Molecular Weight54.1; 45.0; 25.0
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

THNSL2; threonine synthase-like 2 (S. cerevisiae); FLJ10916; FLJ35504; SOFAT; THS2; TSH2; OTTHUMP00000160830; OTTHUMP00000160831; secreted osteoclastogenic factor of activated T cells; threonine synthase-like 2

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

THNSL2 Antibody (orb79347)

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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