THBS3 Antibody

SKU: orb20159

Description

Goat polyclonal antibody to THBS3

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 1-2µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 50kDa band in Human Lung and in lysates of cell line A549 after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to NP_009043.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: THBS3
• Molecular Weight: 104
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti THBS3 antibody, anti thrombospondin 3 antibody, anti MGC119564 antibody, anti MGC119565 antibody, anti TSP3 antibody, anti OTTHUMP00000033827 antibody

Immunohistochemical staining of Human Colon using THBS3 antibody

Quick Database Links

Gene Symbol

THBS3

NCBI Gene ID

7059