Goat anti-TCF3 / ITF1 Antibody

• Catalog Number: orb19418
• Category: Antibodies
• Description: Goat polyclonal antibody to TCF3
• Target: TCF3 / ITF1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KAPRARTSPDEDED
• RRID: AB_10755709
• MW: 67.6
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5ug/ml.WB: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). Recommended concentration: 0.01-0.03 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TCF3 antibody, anti ITF1 antibody, anti trans
• Note: For research use only
• Entrez: 6929

orb19418 (0.01ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19418 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.