TCF2/VHNF1 Antibody

SKU: orb19402

Description

Goat polyclonal antibody to HNF1B

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat, Zebrafish
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 100kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TCF2 / VHNF1
• Protein Sequence: QAYDRQKNPSKEER
• Molecular Weight: 61.3; 58.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TCF2 antibody, anti VHNF1 antibody, anti transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor antibody, anti FJHN antibody, anti HNF1B antibody, anti HNF1beta antibody, anti HNF2 antibody, anti LFB3 antibody, anti MODY5 antibody, anti transcription factor 2 antibody

orb19402 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

TCF2 / VHNF1

NCBI Gene ID

6928