SYNGAP1 (aa1169-1183) Antibody

• Catalog Number: orb125221
• Category: Antibodies
• Description: Goat polyclonal antibody to SYNGAP1
• Target: SYNGAP1 (aa1169-1183)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ESAHIEREEYKLKEY
• MW: 148
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.03-0.1 μg/ml
• Application notes: WB: Approx 150kDa band observed in Human Cerebral Cortex and in Mouse Brain lysates (calculated MW of 148kDa according to Human NP_006763.2 and Mouse XP_990642.3). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 4µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in Neuro-2a, A431 and U251 cells. Recommended concentration: 10µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DKFZp761G1421 antibody, anti KIAA1938 antibod
• Research Area: Cancer, Neuroscience
• Note: For research use only
• Entrez: 8831

Western blot analysis of Mouse Brain lysate using SYNGAP1 antibody