STX6 Antibody

SKU: orb19226

Description

Goat polyclonal antibody to STX6

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 30kDa band observed in Human Lymph Node lysates (calculated MW of 29.2kDa according to NP_005810.1). In transfected HEK293 transiently expressing Human STX6 a band of approx. 30kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: STX6
• Protein Sequence: QALAERKNRQA
• Molecular Weight: 29.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti STX6 antibody, anti syntaxin 6 antibody, anti HGNC:11441 antibody

orb19226 (0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Human STX6 (RC202951) and probed with orb19226 (mock transfection in first lane), tested by Origene.

Quick Database Links

Gene Symbol

STX6

NCBI Gene ID

10228