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Catalog Number | orb19226 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to STX6 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 29.2 |
Target | STX6 |
Entrez | 10228 |
Protein Sequence | QALAERKNRQA |
RRID | AB_10748667 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti STX6 antibody, anti syntaxin 6 antibody, anti Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 30kDa band observed in Human Lymph Node lysates (calculated MW of 29.2kDa according to NP_005810.1). In transfected HEK293 transiently expressing Human STX6 a band of approx. 30kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19226 (0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Human STX6 (RC202951) and probed with orb19226 (mock transfection in first lane), tested by Origene.
WB | |
Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep | |
Goat | |
Polyclonal | |
Unconjugated |