Goat anti-STAT3 (isoform 1 and 2) Antibody

• Catalog Number: orb18540
• Category: Antibodies
• Description: Goat polyclonal antibody to STAT3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Bovine, Human, Mouse, Rat
• Concentration: < p>4-8 μg

• Dilution range: ELISA: 1:8000, WB: 2 μg/ml, IHC-P: 4-6 μg/ml
• Conjugation: Unconjugated
• MW: 88.0; 88.1
• Target: STAT3 (isoform 1 and 2)
• Entrez: 6774
• Protein Sequence: DMELTSECATSPM
• RRID: AB_10755560
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti STAT3 antibody, anti signal transducer and ac
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IE: An anonymous customer found positive results in IF on Human HEK 293 cells. IF; An anonymous customer found positive results in IF on Rat glial processes and nuclei.IHC: In paraffin embedded Mouse Thymus shows preferential staining of the medula over the cortex. Recommended concentration, 4-8µg/ml.WB: Approx. 90kDa band observed in lysates of NIH3T3 (calculated MW of 88.0kDa according to NP_644805.1 and of 88.1 according to NP_003141.2). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

7 µg/ml staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.