Spondin 2/Mindin Antibody

SKU: orb20563

Description

Goat polyclonal antibody to SPON2

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:16000, WB: 1 μg/ml
• Predicted Reactivity: Bovine, Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 35kDa band observed in Human Prostate lysates (calculated MW of 35.8kDa according to NP_036577.1). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Spondin 2 / Mindin
• Protein Sequence: RKNQYVSNGLRDFA
• Molecular Weight: 35.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DIL1 antibody, anti DIL-1 antibody, anti Mindin antibody, anti M-spondin antibody, anti spondin 2, extracellular matrix protein antibody, anti SPON2 antibody

orb20563 (1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Spondin 2 / Mindin

NCBI Gene ID

10417