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Goat anti-SPA1 / SIPA1 Antibody

Catalog Number: orb18646

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb18646
CategoryAntibodies
DescriptionGoat polyclonal antibody to SIPA1
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman
Dilution rangeELISA: 1:32000, WB: 1-3 μg/ml
ConjugationUnconjugated
MW112
TargetSPA1 / SIPA1
Entrez6494
Protein SequenceASKQLGSPTADLA
RRIDAB_10752524
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti SIPA1 antibody, anti SPA1 antibody, anti MGC1
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272(44):28081-8; PMID: 9346962). Recommended concentration: 1-3 μg/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.
Expiration Date12 months from date of receipt.
Goat anti-SPA1 / SIPA1 Antibody

orb18646 staining (1ug/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.