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Goat anti-SPA1 / SIPA1 Antibody
Catalog Number: orb18646
| Catalog Number | orb18646 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to SIPA1 |
| Target | SPA1 / SIPA1 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Protein Sequence | ASKQLGSPTADLA |
| RRID | AB_10752524 |
| MW | 112 |
| Tested applications | ELISA, WB |
| Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272(44):28081-8; PMID: 9346962). Recommended concentration: 1-3 μg/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti SIPA1 antibody, anti SPA1 antibody, anti MGC1 Read more... |
| Note | For research use only |
| Entrez | 6494 |
orb18646 staining (1ug/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
