Somatostatin proprotein (aa58-70) Antibody

SKU: orb668961

Description

Goat polyclonal antibody to SST

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IF
• Dilution Range: ELISA: 1:1000, IF: 10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Application Notes:
  Notes: Immunofluorescence: Strong expression of the protein seen in the plasma membrane and endoplasmic reticulum of U2OS and U251 cells. Recommended concentration: 10µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Somatostatin proprotein (aa58-70)
• Protein Sequence: SEPNQTENDALEP
• Molecular Weight: 12.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SST; somatostatin; SMST; growth hormone release-inhibiting factor; somatostatin-14; somatostatin-28

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

Gene Symbol

Somatostatin proprotein (aa58-70)

NCBI Gene ID

6750