Goat anti-SOCS7 Antibody

• Catalog Number: orb20107
• Category: Antibodies
• Description: Goat polyclonal antibody to SOCS7
• Target: SOCS7
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DAGRKPKLTRTQS
• RRID: AB_10752065
• MW: 63
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 60kDa band observed in lysates of Daudi (calculated MW of 63.0kDa according to NP_055413.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SOCS7 antibody, anti suppressor of cytokine s
• Note: For research use only
• Entrez: 30837
• Expiration Date: 12 months from date of receipt.

orb20107 (1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.