Goat SMC1 Antibody

SKU: orb1524097

Description

SMC1 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1183 and C-terminus
• Target: SMC1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDLS2; DXS423E; epididymis secretory sperm binding protein; SB1.8; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC-1A; SMC1alpha; SMC-1-alpha; SMC1L1; SMCB; structural maintenance of chromosomes protein 1A

Detection of mouse SMC1 by western blot. Samples: Whole cell lysate (15 and 50 µg) from NIH 3T3 cells. Antibodies: Affinity purified goat anti-SMC1 antibody orb1524097 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human SMC1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (H; 50 µg) , and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified goat anti-SMC1 antibody orb1524097 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified goat anti-SMC1 antibody orb1524097 used for IP at 6 µg/mg lysate.

Detection of human SMC1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-SMC1 (Cat. No. orb1524097) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse SMC1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified goat anti-SMC1 (Cat. No. orb1524097) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SMC1

UniProt

Q14683

RefSeq (Protein):NP_006297.2