SMARCA3 Antibody

• Catalog Number: orb18396
• Category: Antibodies
• Description: Goat polyclonal antibody to HLTF
• Target: SMARCA3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KQAKINEIRTLIDL
• RRID: AB_10750923
• MW: 114
• Tested applications: ELISA, FC, WB
• Dilution range: ELISA: 1:16000, WB: 0.2-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 120kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 0.2-1 μg/ml. Please note that the weaker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observations (J Biol Chem 1995 Mar 3;270(9):4575-87).
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HLTF, SMARCA3, ZBU1, HLTF1, RNF80, HIP116, SNF2L3,
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 6596
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Jurkat lysate using HLTF antibody