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SMARCA3 Antibody

SKU: orb18396

Description

Goat polyclonal antibody to HLTF

Images & Validation

Tested ApplicationsELISA, FC, WB
Dilution rangeELISA: 1:16000, WB: 0.2-1 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 120kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 0.2-1 μg/ml. Please note that the weaker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observations (J Biol Chem 1995 Mar 3;270(9):4575-87).

Key Properties

HostGoat
ClonalityPolyclonal
TargetSMARCA3
Molecular Weight114
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

HLTF, SMARCA3, ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3, SNF2-like 3, sucrose nonfermenting-like 3, helicase-like transcription factor, DNA-binding prote

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SMARCA3 Antibody

Western blot analysis of Jurkat lysate using HLTF antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

SMARCA3 Antibody (orb18396)

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Available Sizes

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100 μg
$ 500.00
DispatchUsually dispatched within 5-10 working days
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