Slc10a2 (mouse) Antibody

SKU: orb19974

Description

Goat polyclonal antibody to Slc10a2*

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx. 35kDa band observed in Mouse Liver lysates (calculated MW of 38.1kDa according to NP_035518.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Mouse Small Intestine. Recommended concentration: 5µg/ml.
• Reactivity: Mouse

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Slc10a2 (mouse)
• Protein Sequence: DETNKGFQPDEK
• Molecular Weight: 38.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Slc10a2 antibody, anti solute carrier family 10 (sodium/bile acid cotransporter family), member 2 antibody, anti ASBT antibody, anti ISBT antibody, anti ileal apical sodium-dependent bile acid transporter antibody, anti ileal sodium-dependent bile acid transporter antibody, anti solute carrier family 10, member 2 antibody

Primary incubation 1 hour at room temperature. Image A: Mouse Liver lysate at primary Ab concentration 0.3 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Mouse Small Intestine. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Mouse Small Intestine, with no primary antibody.

Quick Database Links

Gene Symbol

Slc10a2 (mouse)