Goat anti-Sirtuin 3 (mouse) Antibody

• Catalog Number: orb22501
• Category: Antibodies
• Description: Goat Polyclonal to SIRT3
• Target: Sirtuin 3 (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RASGIPASKLVEAH
• RRID: AB_10922720
• MW: 28.8; 36.6
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:64000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Application notes: WB: Approx 30kDa band observed in Mouse Heart lysates (calculated MW of 28.8kDa according to NP_071878.2). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti -SIR2-like protein 3 antibody, anti-OTTMUSP00
• Note: For research use only
• Entrez: 23410

orb22501 (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of NIH3T3 cells using SIRT3 antibody

Flow cytometric analysis of NIH3T3 cells using SIRT3 antibody