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Sirtuin 3 (mouse) Antibody

SKU: orb22501

Description

Goat Polyclonal to SIRT3

Research Area

Cardiovascular Disease

Images & Validation

Tested ApplicationsELISA, FC, IF, WB
Dilution RangeELISA: 1:64000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
ReactivityMouse
Predicted ReactivityHuman, Porcine, Rat
Application Notes
WB: Approx 30kDa band observed in Mouse Heart lysates (calculated MW of 28.8kDa according to NP_071878.2). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

ClonalityPolyclonal
TargetSirtuin 3 (mouse)
Protein SequenceRASGIPASKLVEAH
Molecular Weight28.8; 36.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti -SIR2-like protein 3 antibody, anti-OTTMUSP00000027445 antibody, anti-OTTMUSP00000027444 antibody, anti-OTTMUSP00000027441 antibody, anti-NAD-dependent deacetylase sirtuin-3 antibody, anti-Sir2l3 antibody, anti-AI848213 antibody, anti-2310003L23Rik antibody, anti-homolog) 3 antibody, anti-sirtuin 3 (silent mating type information regulation 2 antibody, anti-sirtuin 3 antibody, anti-Sirt3 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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