Goat anti-Sirtuin 3 (mouse) Antibody

• Catalog Number: orb22501
• Category: Antibodies
• Description: Goat Polyclonal to SIRT3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:64000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 28.8; 36.6
• Target: Sirtuin 3 (mouse)
• Entrez: 23410
• Protein Sequence: RASGIPASKLVEAH
• RRID: AB_10922720
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti -SIR2-like protein 3 antibody, anti-OTTMUSP00
• Note: For research use only
• Application notes: WB: Approx 30kDa band observed in Mouse Heart lysates (calculated MW of 28.8kDa according to NP_071878.2). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb22501 (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of NIH3T3 cells using SIRT3 antibody

Flow cytometric analysis of NIH3T3 cells using SIRT3 antibody