Sirtuin 1/SIRT1 (aa577-590) Antibody

SKU: orb22540

Description

Goat Polyclonal to SIRT1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.05-0.2 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  WB: Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.05-0.2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Sirtuin 1 / SIRT1 (aa577-590)
• Protein Sequence: EKPQEVQTSRNVES
• Molecular Weight: 81.7; 50.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti sirtuin type 1 antibody, sir2-like 1 antibody, hSIR2 antibody, SIR2alpha antibody, SIR2-like protein 1 antibody, OTTHUMP00000198112 antibody, OTTHUMP00000198111 antibody, NAD-dependent deacetylase sirtuin-1 antibody, SIR2L1 antibody, sirtuin 1 antibody, SIRT1 antibody

Immunohistochemical analysis of formalin-fixed and paraffin embedded human Skin tissue using SIRT1 antibody

Western blot analysis of Jurkat cell lysate using SIRT1 antibody (35ug)

Quick Database Links

Gene Symbol

Sirtuin 1 / SIRT1 (aa577-590)

NCBI Gene ID

23411