SIRT1 Antibody

SKU: orb19330

Description

Goat polyclonal antibody to SIRT1

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 1-3 μg/ml
• Reactivity: Mouse
• Predicted Reactivity: Canine, Human, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 120kDa band observed in nuclear lysates of HeLa (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SIRT1
• Protein Sequence: EITEKPPRTQKE
• Molecular Weight: 81.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SIRT1 antibody, anti sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody, anti RP11-57G10.3 antibody, anti SIR2L1 antibody, anti OTTHUMP00000060745 antibody, anti SIR2alpha antibody, anti sir2-like 1 antibody, anti sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1 antibody, anti sirtuin 1 antibody, anti sirtuin type 1 antibody

Western blot analysis of NIH3T3 cell lysate using SIRT1 antibody.

Quick Database Links

Gene Symbol

SIRT1

NCBI Gene ID

23411