Goat anti-SIRT1 Antibody

• Catalog Number: orb19330
• Category: Antibodies
• Description: Goat polyclonal antibody to SIRT1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 81.7
• Target: SIRT1
• Entrez: 23411
• Protein Sequence: EITEKPPRTQKE
• RRID: AB_10753327
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SIRT1 antibody, anti sirtuin (silent mating t
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 120kDa band observed in nuclear lysates of HeLa (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NIH3T3 cell lysate using SIRT1 antibody.