SFRP4 Antibody

SKU: orb20135

Description

Goat polyclonal antibody against SFRP4

Research Area

Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways, Stem Cells, Wnt

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-4µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 40kDa band observed in Human Uterus lysates (calculated MW of 39.8kDa according to NP_003005.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SFRP4
• Protein Sequence: TNSSCQCPHILPHQD
• Molecular Weight: 39.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-FRP 4 antibody, anti-FRP4 antibody, anti-sFRP-4 antibody, anti-Sfrp4 antibody, anti-FrpHE antibody, anti-human endometrium antibody, anti-Secreted frizzled-related protein 4 antibody, anti-Frizzled protein antibody, anti-Frizzled protein human endometrium antibody

Immunohistochemical analysis of paraffin embedded Human Uterus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining with SFRP4 antibody.

Western blot analysis of staining of Human Uterus lysate (35µg protein in RIPA buffer) using SFRP4 antibody.

Quick Database Links

Gene Symbol

SFRP4

NCBI Gene ID

6424