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Serotonin receptor 1B/HTR1B Antibody

SKU: orb19283

Description

Goat polyclonal antibody to HTR1B

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:128000, WB: 1-3 μg/ml
ReactivityHuman, Mouse
Predicted ReactivityBovine, Canine, Feline, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 48kDa band observed in Human Brain lysates (calculated MW of 43.6kDa according to NP_000854.1). Recommended concentration: 1-3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetSerotonin receptor 1B / HTR1B
Protein SequenceSNEDFKQAFHK
Molecular Weight43.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti HTR1B antibody, anti 5-hydroxytryptamine (serotonin) receptor 1B antibody, anti 5-HT1B antibody, anti 5-HT1DB antibody, anti HTR1D2 antibody, anti HTR1DB antibody, anti S12 antibody

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Serotonin receptor 1B/HTR1B Antibody

orb19283 (2ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Serotonin receptor 1B/HTR1B Antibody

Western blot analysis of Human Cerebellum (Lane 1) and Mouse spinal cord (Lane 2) lysates using HTR1B antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Serotonin receptor 1B/HTR1B Antibody (orb19283)

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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