Goat anti-SEMA3E Antibody

SKU: orb18970

Description

Goat polyclonal antibody to SEMA3E

Research Area

Cancer, Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:64000, WB: 0.1 μg/ml, IHC-P: 2-4µg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Adrenal Medulla shows granular cytoplasmic staining increasing towards the zona reticularis. Recommended concentration, 2-4ug/ml.WB: Preliminary experiments gave bands at approx 35kDa and 20kDa in Human Prostate lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 89.2kDa according to NP_036563.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SEMA3E
• Protein Sequence: KPEHYRLPRHTLDS
• Molecular Weight: 89.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SEMA3E antibody, anti SEMAH antibody, anti coll-5 antibody, anti M-SEMAH antibody, anti M-SemaK antibody, anti KIAA0331 antibody, anti M-sema H antibody, anti sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E antibody, anti sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E antibody

orb18970 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb18970 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb18970 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, PM and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb18970 (2.5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb18970 (2.5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

SEMA3E

NCBI Gene ID

9723